First, the cell line
Cell Strain: That is to say, a cell strain is a cell population formed by single cell proliferation or by a single cell proliferation or by screening. The specific nature or signature of the cell line must be present throughout the culture period. The primary culture is the cell line after successful first passage, and consists of the cell lineage originally present in the primary culture. If the passage cannot be continued, or the number of passages is limited, it can be called a finite cell line. If it can be continuously cultured, it is called a continuous cell line, cultured for more than 50 generations and cultured indefinitely. Therefore, a cell line is a cultured cell having a special property or a marker obtained from a primary culture or a cell line by a selection method or a colony formation method. In terms of culture algebra, it can be cultured to 40-50 generations. The specific nature or signature of the cell line must be present throughout the culture period. For human tumor cells, cultured in vitro for more than half a year, stable growth, and serial passage can be called a continuous strain or line.
Second, the cell line
Cell line refers to a population of cells in which primary cell cultures are propagated after successful first passage. Also refers to cultured cells that can be serially passaged for a long period of time. (Therefore, the subsequent limited cell line (FiniteCellLine), Infinite Cell Line ) was introduced. Therefore, the cell line narrowly refers to cells that can be serially passaged (both spoken and written in a specific environment), in a broad sense. Refers to cells that can be passaged
The cells that passed the second death crisis after 40-50 divisions are called cell lines. If the cell line has a limited lifetime , it is called a finite cell line (FiniteCellLine); a cell line that has been infinitely propagated, called a continuous or infinite cell line. (InfiniteCellLine). Most of the infinite cell lines have undergone heteroploidization, have aneuploid karyotype , and may become malignant cells, and thus are essentially transformed cell lines. The infinite cell line has immortality (undead), but still retains contact inhibition and no inoculation to death; some have immortality, and allogeneic inoculation has tumorigenicity, indicating that it has been malignant. A cell line isolated from a certain cell line and having a different trait from the original cell line is called a subline.
the difference
The primary cultured cells are generally not passed down until about 10 generations, and the growth of the cells will be stagnant, and most of the cells will die of aging. However, a very small number of cells can pass through the "crisis" and continue to pass on. These surviving cells can generally be passed to 40-50 generations. This passage cell is called a cell strain. The genetic material of the cell line did not change. When the cells pass to the 50th generation, there will be a "crisis" that cannot be passed down. However, some of the cells have changed their genetic material and are characterized by carcinogenesis. They may be passed down without restriction under culture conditions. This passage cell is called a cell line. In some other sources, the concept of cell lines and cell lines is exactly the opposite of what we say in our textbooks. The concept of the textbook does not introduce this concept. This is why? Bcying, the editor of the People's Education Society, explained this doubt: cell lines and cell lines, which were once mixed, led to some unclear concepts in some literature. The concepts referred to below are more common at home and abroad, and are accepted by most researchers.
Primary cultures are called cell lines after successful passage for the first time , so cell lines can refer to cells that are likely to be passaged. Among the cells that can be serially passaged are called continuous cell lines or infinite cell lines , and those that cannot be continuously cultured are called finite cell lines . Most diploid cells are finite cell lines . A culture having a specific property or marker obtained from a primary culture or cell line by a selection method or a clone formation method is called a cell strain (CellStrain), that is, the cell strain is isolated or cultured by single cells or screened. Method, a population of cells formed by single cell proliferation. The specific nature or signature of the cell line must be present throughout the culture period.
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