After picking up the story, Xiao Bian introduced to the complex samples, how to use the black technology of liquid chromatography to do "fancy" separation. With the "fancy" separation of the front end, it is necessary to have a "fancy" test. What is the Transformers? What dinosaur team is, the fancy + combination becomes stronger after the combination...
So in this issue, Xiaobian talks to you about the combination of "fancy" separation "fancy detection"--how to analyze the compound "fancy" by multiple mass spectrometry.
As a product with 50 years of mass spectrometry, Thermo Scientific's mass spectrometry ranges from organic mass spectrometry to inorganic mass spectrometry, from liquid to gas, from single quadrupole to tandem quadrupole, from ion trap, high resolution magnetic mass spectrometry to high resolution. Orbitrap; from single quadrupole ICP-MS to tandem quadrupole ICP-MS, from high resolution ICP-MS to featured inorganic isotope series mass spectrometry. Numerous mass spectrometry techniques and the patents obtained are full of hanging walls. If they come together, I am afraid that it will not be able to close the eyes for a few days and nights. Here, for the "fancy" interpretation of organic compounds, Xiaobian first introduces the "carrier" level of the organic mass spectrometer - Orbitrap Fusion.
Orbitrap Fusion TM Lumos TM Tribrid TM 3-in-1 Mass Spectrometer
As an artifact, the Orbitrap Fusion series of mass spectrometers are equipped with full black technology, and only three types of mass analyzers are used: Orbitrap electrostatic field orbitrap, dual-pressure linear ion trap and hyperbolic quadrupole.
Orbitrap electrostatic field orbitrap high-resolution mass spectrometry combines the advantages of ultra-high resolution, high-quality precision, and high sensitivity, and can now achieve an ultra-high resolution of 1 million. What is the use of such a high resolution? As an example, a general resolution of mass spectrometry and ultra-high resolution mass spectrometry is like walking in a heavily polluted haze and a sunny, clean air environment (if you live in a well-ventilated area) Imagine the difference between looking at things when the glasses are full of fog and when the lenses are clean. If you are not nearsighted, Xiaobian can only ask you to use your own imagination...).
Ultra-high resolution helps us to see the information in complex samples more clearly, even if there are no separate components at the chromatographic level, they can make them "out of shape". For another example (as shown below), in the complex matrix, we found the information of timolol at a resolution of 120,000, but when the resolution increased to more than 500,000, we found a component that was originally thought to be It also contains another component, ethyl phenyl ketobase. So high resolution can help us discover more science more realistically.
Let's talk about the double-pressure linear ion trap. The advantage of the ion trap is that it can do multi-stage mass spectrometry and get more detailed structural information of the compound, so when we encounter the components of complex structures, we can use ion trap technology to fully analyze the structure of the compound. Thermo Fisher is also a real "senior" in ion trap technology. The dual-pressure linear ion trap consists of a high-pressure well and a low-pressure well. The high helium pressure in the high-pressure trap enables better ion trapping, cooling, and fragmentation. The low helium pressure in the low-pressure trap is better for mass scanning. Resolution or faster, dual ion traps Each well can be set to optimal helium pressure for optimal capture, isolation, fragmentation and scanning.
In order to get more information about the structure of the compound, Orbitrap Fusion not only has a "high x lattice" ion trap technology, but also has a variety of fragmentation methods, such as CID, HCD, ETD, UVPD and so on. Different fragmentation methods can provide information on the structural fragmentation of different compounds. These fragmentation methods can also be flexibly combined in the multi-stage mass spectrometry. For the compound "fancy" forging, the compound does not reveal the "truth".
Finally, let's talk about the hyperbolic quadrupole. Hyperbolic quadrupoles are more difficult to machine than cylindrical quadrupoles, can achieve higher resolution, and have better selection of ions, especially when doing complex sample analysis. This technology is also used in Thermo Scientific's triple quadrupole mass spectrometry, enabling triple quadrupole mass spectrometry to achieve high-resolution SRM (H-SRM) mode, which quantifies target compounds in complex matrix samples. Good sensitivity.
On Orbitrap Fusion, different quality analyzers and different fragmentation methods can be flexibly combined with "fancy" and work together to achieve more ways of working beyond the imagination, and realize more possibilities for cutting-edge research of scientific users. Let's take a simple working mode as an example to experience the "fancy" movement of ions in Orbitrap Fusion.
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Let your scientific highlights shine even more to teach you how to "Buddha" Xiaobai Feisheng "Magic" God of War
Let your scientific highlights shine more "Fancy" interpretation of organic compounds (Part 1)
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