When the hens pay for the calcium production, most eggs are laid in the morning, and the next egg yolk is discharged from the ovary into the fallopian tube half an hour after the egg is laid. Therefore, the hens do not need to be supplemented with calcium in the morning. By 14 o’clock in the afternoon, as the egg shell deposition rate increases, the amount of calcium consumed by chickens will increase. Therefore, in order to make laying hens produce more eggs and produce good eggs, the effect of feeding calcium hens with laying hens is best at 12 to 18 o'clock every day.
Calcium supplementation methods and effects Calcium source feeds can be placed on their own, allowing any chicken to eat freely, or they can be fed mixed with feed. Calcium source feed has a good effect on improving chicken egg production, egg fertilization rate, chick hatching and shelling rate, serum calcium content, and egg shell thickness.
Calcium supplementation The production of laying hens requires calcium supplementation, but it is not always better. For a layer of eggs, 4.4 grams of calcium is required for each egg. The calcium content in the diet is 3.0%-3.5% to meet the production needs of the layer.
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Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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