The aseptic operation technical regulations for the inoculation of boletus vaccination in the aseptic operation are mainly in the following five aspects.
1 Select the time to choose sunny day midnight or early morning vaccination. At this time, the temperature is low and the bacteria are in a dormant state, which is conducive to improving the yield of the bag inoculation.
2 After the inoculum enters the room plastic bag and moves into the sterile room or the inoculation account, the second disinfection is carried out together with the strain, the inoculation tool and the alcohol lamp. First use aerosol to smoke for more than 30 minutes, 40-60 minutes before inoculation, and then irradiate with ultraviolet light for 30 minutes to achieve aseptic conditions.
3 Inoculate the agile bag material and open the bag to tie the rope. If it is the collar cotton plug, pull out the cotton plug. Put the strain into the bag, and re-attach the bag or cotton plug to restore the seal. If the bottle is covered, the bottle cover film is peeled off and recovered after inoculation.
4 update air Each batch of bags is inoculated, you must open the doors and windows to ventilate for 30-40 minutes, then close the doors and windows, re-sterilize, continue to inoculate.
5 Cleaning up the residue During the inoculation process, the cover film waste of the strain bottle, especially the wood chips on the workbench and the indoor site, must be concentrated in one corner and not thrown away. After the inoculation of each batch of bags, combined with ventilation and ventilation, a cleaning is carried out to keep the site clean and prevent contamination of the bacteria.
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