With the rapid development of black fungus bags, the traditional use of sawdust as a culture material has been far from meeting local needs. The research group of the Center for the Promotion of Edible Medicinal Fungi of Linkou County, Linkou County, Heilongjiang Province, succeeded in using leaves instead of sawdust cuttings to cultivate black fungus. The invention of this new technology will produce incalculable economic and social benefits. The technical points are described below.
First, deal with raw materials of broadleaf leaves. Collect the leaves and twigs that fall from the forest area and return them to the sun and crush them. Each crushed 30 kg of dry leaves, while crushed 10 kg of bean straw spare.
Second, the culture formula and preparation methods 1 cultivation materials formula: leaf powder 71.7%, bean straw powder 20%, corn flour 5%, soybean powder 2%, gypsum 0.6%, lime powder 0.7%, add water to adjust the moisture content 55% , pH 7-8.5.
2 Preparation method: Weigh the required amount of dry leaf powder on the cement floor, and spread the bean straw powder into the leaf powder (dry material) and mix well. Spread corn flour and soy flour over the leaf powder and mix well. Gypsum and white ash were then melted with warm water and sprinkled on the wet dry material. After mixing the wet material of the culture material, the material shall be sieved in two times with a coarse wire sieve to make the thickness of the culture material uniform, and the water content shall be 55%. That is to say, the material is used to dip the material, and there is a moist feeling on the hand surface and there is no water retention.
Third, bagging, sterilization and access to bacteria 1 bagging: culture materials bagging, bag selection 17 cm 33 cm 4.5 meters thick polyethylene bags. Side loading material compaction, so that the culture medium in the bag is slightly tighter, but do not bulge the breakage bag. Put 9 cm into the mouth of the bag. After the material surface is flattened and compacted, use a round iron bar with a diameter of 2.5 cm to tie a deep eye in the middle of the bag. The depth should be 2 cm from the bottom of the bag. Pour out the bulk material that comes out of your eyes and prevent the bulk material from blocking the eyes. Then close the bag mouth together and press down slightly (excluding the air inside the bag). Hold it tightly and hold it tightly and turn it clockwise. Then use your fingers to hold the screwed bag in place. Place it upside down on a dense iron basket or wooden steamer curtain (each basket or board) Put 2-3 layers of bags on the curtains.)
2 Atmospheric pressure sterilization: The material bag is loaded into the pot and the pot door is closed, and the fire is heated to rapidly bring the temperature of the steamer to 100°C. Insulation, sterilization after 6-8 hours ceasefire. Continue to steam in the steamer overnight. The next morning the pan. (In the case of water shortage in the middle of the atmospheric pressure cooker, add boiling water above 90°C. Prolong the sterilization for half an hour for each additional boiling water). When out, pot and carry, it is required that both hands hold the middle of the bag.
3 Inoculation: Use a thick metal skin to make a drum slightly larger than the electric furnace. The barrel is 35 centimeters high. Put it on the electric furnace. Place a thin layer of barbed wire on the drum and fix it. (Wire mesh is used to prevent inoculation of bacteria. Fall into the furnace to produce smoke). When the temperature of the bag falls to 28°C, warm inoculation is performed in the aseptic zone above the drum of the electric furnace. During the inoculation, the operation is required to be light to prevent the wind from hitting the heat on the barrel. Each bottle of original species can transfer 25-30 bags of cultivars.
Fourth, the treatment of bacteria chamber and mycelium culture 1 bacteria treatment: The indoor debris is cleaned up, the training room walls require a smooth and smooth, painted again indoor, with a dry wooden rod erected fungus frame. The room temperature was raised to 30°C and maintained for 24 hours to rejuvenate the spores in the room. The 0.2% carbendazim solution was used to spray the wall and the bacteria holders in the room and the indoor air humidity was maintained at 75%. Each cubic meter of space is fumigated with 15 grams of sulphur, killing the revived germs, and finally heating the interior walls and the bacteria rack.
2 Hyphae culture: After the strainer has finished the strains, it is immediately moved to the rack of the culture room. The pockets of the bags face upward, and the interiors are kept dark. Do not place the bags too tight between the bags. The germination and colonization period was 5-10 days before germination, and 28°C was appropriate at room temperature. This period does not require ventilation, the temperature can not exceed 30 °C, when the mycelium grows to 1/3, the indoor temperature drops to 24 °C. Checked once a day and found that the bacteria immediately took the bag out of the culturing room. White ash is often sprinkled on the ground to dry the room. Bacteria come to the middle and late, when the room temperature should be controlled at 18-22 °C. And often ventilated. 35-40 days mycelium can be full bag. After that, with the temperature difference stimulus, see the light to continue cultivation for 10 days so that the hyphae can be fully eaten.
Fifth, the greenhouse three-dimensional hanging bag ear management use simple greenhouse three-dimensional hanging bag cultivation of black fungus with insulation, moisture retention, easy management, small footprint, cultivation of many advantages. First, a simple wooden ear bar (2 meters high, 6 meters wide, 15 meters long, and 10,000 three-dimensional hanging bags) can be used. In the middle of the ear canopy, a 1 meter wide culture management road is left, and a 10th wire is pulled every 25 centimeters on the shed, and the two heads are fixed and used for hanging the ropes. The four sides and the roof of the scaffolding are surrounded by straw curtains or crop straws. The ground is first sprayed with a 0.2% carbendazim solution, slightly dry, and then disinfected with 1 layer of white ash. After the bacteria bags were all fermented, they were moved into the culture shed, and the surfaces of the bacteria bags were scrubbed with 0.2% potassium permanganate solution. The bacteria bags were tied to the wire ropes. After hanging one rope, the mouth was cut with a disinfectant blade. Each bag cuts 5 long vertical bars at intervals and cuts 2 small short ports at the bottom of the bag. After all the bags have been hung and cut, the shed temperature is maintained at 20°C during the earing period, and the water is sprayed 2-3 times a day on the ground. Water is often sprayed on the grass curtains outside the greenhouse to maintain the relative humidity of the air in the shed. , calm wind cultivation. The primordium was formed from the cut in 7-10 days. When the ear buds are sealed at the cut mouth, the water can be directly sprayed on the bag (this time, the relative humidity of the air is 90-95%). Temperature cannot exceed 26°C. Due to the large number of three-dimensional hanging bags in the greenhouse, the upper layer of bacteria bags is required to spray more and spray more frequently, and the lower layer of bacteria bags requires less spray and micro spray. The amount of water spray should be controlled flexibly, but no water can be sprayed when the temperature is high at noon. Do not flatten the flatness of the ear, and the glossy and moist feeling is the best standard.
In short, the ear does not need to spray water without curling, black fungus grow to middle and late, should maintain fresh air circulation in the shed, cloudy amount of less spray, less rain or spray. When the temperature is high and the humidity is high, more attention should be paid to the ventilation and ventilation. This is not only beneficial to the growth of black fungus, but also an effective measure to prevent the contamination of bacteria and the ear damage. It took 30 days from harvesting ear to mature harvesting. This culture material can produce 60-70 grams dry ears per bag. When the fungus matures, it picks when it is sunny. The harvested fungus was timely distributed on the ventilated curtains, and the ears were turned in once when exposed to semi-dryness. Generally, they were dried and stored in 2 days.
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