In order to solve the problem of pests in rapeseed, a major oil crop in China, to increase rapeseed yield, reduce the use of pesticides, and protect the ecological environment, the research team led by Professor Chunyun Guan has adopted genetic engineering technology to transfer Bt toxin genes after 4 years of research. In rapeseed cells, rapeseeds are resistant to insects and stably inherited and expressed in oilseed rape. Academicians Fan Yunliu, Fu Tingdong, Yuan Longping of the Chinese Academy of Engineering and experts from the Chinese Academy of Agricultural Sciences, Hunan Academy of Agricultural Sciences, Huazhong Agricultural University, Hunan Normal University, and the Hunan Provincial Department of Agriculture believe that the research topic is correct and the experimental results are reliable. The rapeseed strain was the first Bt-resistant insect-resistant rape strain in China, and was also the first genetically modified crop in Hunan Province. Rape is the main oil crop in China, and its planting area and total output rank first in the world. However, the output of China's oil menu is not high. The reason is that pests and diseases are one of the important factors. Pieris rapae is a major pest that harms the rapeseed, overlapping generations of hazards, severe rape leaves only leaves veins and petioles, seriously affecting the yield, autumn rain in many areas often lead to disaster. In the past, control of cabbage caterpillars was mainly controlled by pesticides such as trichlorfon and imine sulfuric acid. Due to the strong drug resistance of the cabbage caterpillar, the use of drug control is generally more effective for the 3rd instar larvae, but less effective for the 3rd instar larvae, especially chemical pesticides not only pollute the environment, but also increase the cost. As for insect-resistant breeding, basically no development was carried out because no source was found. In 1988, foreign scholars adopted genetic engineering methods to obtain the first generation of Bt insect-resistant plants, and the real Bt transgenic plants were the second-generation Bt insect-resistant plants, such as the tobacco-resistant genes obtained by Belgian experts. For more than a decade, domestic and foreign scientists have obtained rice, corn, cotton, and tomato plants that have been transformed into Bt-resistant insect-resistant plants. Some scholars in China have also obtained transgenic mustard plants from Brassica juncea and Brassica napus, but none have yet been obtained. Stable material reports. Since 1996, Prof. Chunyun Chun and others have undertaken the two topics of “High-tech agricultural high-tech cultivation of new varieties of crops and its supporting technologies†and “Utilizing genetic engineering technology to develop new varieties of rapeseed†in order to cultivate themselves successfully and The “Xianyou 13â€, a double-low rapeseed variety that has been listed by the government as the “Ninth Five-year Plan†promotion, was used as a genetic transformation receptor. Bt toxin gene was introduced into the plant through ovary injection and Agrobacterium-mediated transformation to obtain transgenic plants. Among them, a transgenic insect-resistant rape strain was obtained by ovary injection. Practice has proved that this strain has obvious insect resistance to Pieris rapae, and the survival rate of Pieris rapae is significantly reduced, less than 20%, while the control survival rate is as high as 70%. In addition, the feeding speed of P. rapae decreased, and the weight of adult pupae decreased, and the larvae of middle-aged and older larvae prolonged. The insect-resistant advantages of the lines were significant. Prof. Chunyun Chun said that the next phase of the study will focus on the safety of the strain, its impact on the pollination environment of the crop, the stability of the insect resistance, and the impact of the strain on bees in the pollination media. After approval by the Agricultural Bioengineering Safety Committee, more experiments were conducted to participate in regional trials and product release.
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