Recently, Shandong Baolai Lilai Bioengineering Co., Ltd. has launched a new biological functional feed protein source, biological E protein, which has attracted people's attention in the aquaculture and feed industries. What is the origin and purpose of this R&D of biotech products? What are the technological innovations and specific features? Can we bring new technical breakthroughs to the breeding and feed industries that are developing in the low-lying state of Fujian? The causes of product development: Biological products such as animal microecological preparations, enzyme preparations, and peptide preparations are hot topics in the field of feed biotechnology development in recent years. There are more single products and fewer complex products. At present, it is not yet common, and users have the choice and application problems at the technical level and practical cost. The urgent need for such issues as improving the general resilience of animals to diseases, improving the digestion and absorption of feed nutrients, improving the safety and quality of animal products, reducing the pollution of the livestock industry to the ecological environment, and the non-benign circulation of the livestock industry. The functional role of biological functional components. In the past seven years, the research, development and application experience of the combination of microbial fermentation engineering, enzyme engineering, genetic engineering, and animal nutrition and medicine have shown that different strains should be fermented separately and one step of fermentation should achieve as many biological functions as possible. According to the needs of animals for the compound of biological functional components, metabolites and carriers are best collected together to reduce post-processing costs, and the dominant components must be quantified by process control and final product compounding. II. Bio-E protein product description: Bio-E protein is the development trend of Polarily company based on animal nutrition technology and bio-engineering technology in recent years, and in recent years, international and domestic animal science and technology circles in molecular biology and nutritional biology. The scientific research results obtained are based on forward-looking and leading biological product design concepts, with molecular nutrition and microecological nutrition as the core areas of product research and development. They are a kind of rich micro-ecology, immune micro-ecology, and anti-infection micro-environment developed and produced. Ecology; Digestive enzymes, anti-nutritional factor enzymes, peptidases, enzyme precursors; Nutritional small peptides, biologically active small peptides, structural small peptides, functional small peptides, carrier peptides; B vitamins, organic acids (acetic acid, butyric acid, etc. ), bacterial oligosaccharides, polysaccharides; bio-organic trace elements (selenium, iron, zinc, etc.); amino acids, peptides, proteins and other biological active products. The Bio-E ProteinTM system was developed by the Polaroid Bioengineering Institute in January 1999. After the completion of the pilot test in mid-2001, the first-generation product was trial-produced. However, the company did not promote it throughout the country, but repeatedly tested its practice. Application effect: In the past two years, Bio-E ProteinTM has been in piglets, finishing pigs, pregnant sows, nursing sows, breeding boars; broilers, ducks, ostriches, quail, meat pigeons; layers, ducks, breeders , Breeding; eel, soft-shelled turtle, prawns, crabs, squid, oysters, shellfish; cows, beef cattle, rabbits, rabbits, rabbits, deer, sheep and other animal species done hundreds of complete test report test. At present, the technical parameters and production process of Bio-E Protein TM are basically determined. There are 9 strains of high-performance strains, 7 fermentative steps are involved in the fermentation (in three factories, different strains are fermented separately), and liquid-solid combined fermentation is used. , liquid fermentation and solid fermentation three fermentation methods, the production of biological peptides also uses a specific enzyme system oriented shear and special protection technology. Technical parameters of biological E protein 5055 product: Probiotics: Total number of viable cells ≥ 5109 CFU/Kg Enzyme preparation: Acid protease ≥ 300u/g Neutral protease ≥ 500u/g Amylase ≥ 1000u/g Cellulase ≥ 600u/g β- Glucanase ≥500u/g Xylanase ≥600u/g Pectinase ≥25u/g Lipase ≥6.5u/g Phytase ≥36u/g Vitamin: VB29.13mg/kg VB1 21.33mg/kg VB6 0.204mg/kg VC 0.878mg/kg Organic acid: Acetate 0.225g/kg Propionic acid 2.59g/kg Butyric acid 3.487g/kg Volatile fatty acid 6.292g/kg Where micro-ecological bacteria content: Bacillus producing enzyme ≥ 3109CFU/ Kg Bacillus spp. resistant to infection ≥ 3109 CFU/kg lactic acid bacteria ≥ 2109 CFU/kg Acidic acid bacteria ≥ 1109 CFU/kg Yeast ≥ 1108 CFU/kg Amino acids: Lysine 3.14% Methionine 1.18% Aspartate 3.25% Glutamic acid 7.62% Alanine 2.86% Cystine 0.86% Proline 2.56% Serine 2.85% Leucine 4.16% Isoleucine 1.52% Tyrosine 1.25% Phenylalanine 2.46% Threonine 2.07% Histidine 1.28% Arginine 2.97% Proline 3.43% Tryptophan 0.46% Glutamine 3.8% Crude Protein: ≥50% Crude Fiber: ≤8.0% Chicken Metabolizable Energy: 2980 kcal/kg Pig Metabolizable Energy: 4120 kcal/kg , Characteristics and biological function of protein E: ◎ rich on animal growth and development, metabolism, digestion and absorption, immune regulation and other bioactive components play an important role, a combination of scientific and very complete. This is the most basic and important feature of the biological E protein. Avoid the defects of single component insufficiency. ◎Bio-E protein takes into account the fact that the environment, nutrition, environmental stress and the fact that many animals are in a sub-health state in China's aquaculture industry, and therefore focuses on improving the animals' anti-stress ability, non-specific immunity, and maintaining intestinal bacteria. Group balance ability, intestinal mucosal epithelial cell repair ability, intestinal villus development ability, digestive tract pH adjustment ability, electrolyte balance ability, vitamin, trace element supply ability, digestion and absorption ability, etc. ◎Biological E protein emphasizes the balance of various essential amino acids, especially the essential amino acids that are easily lacking in conventional feed ingredients. From the absorption mechanism, a considerable proportion of the amino acids of biological protein E is absorbed in the form of small peptides, and the operation speed is fast and the absorption efficiency is high. high. ◎ Biological E protein can increase the utilization of protein, energy, and minerals by 5-10%, so feed costs can be appropriately reduced. And reduce fecal nitrogen, phosphorus, calcium excretion, reduce fecal odor and harmful gas emissions, thereby reducing environmental pollution. ◎ Biological protein E significantly improves the quality of livestock products, such as increasing the lean meat rate of pigs, round buttocks, good meat quality and strong water holding capacity; improving the quality and color of eggshells, thick and thick egg white, large and bright egg yolks; improving dairy cows Milk production, milk fat, etc. ◎ Animals have a healthy body and few diseases, significantly reduce the use of antibiotics, and reduce drug residues in livestock products. Fourth, biological E protein for the aquaculture and feed industry to bring a series of solutions: 1, replace the imported high-quality fish meal: In the development process of biological E protein, the best natural high-quality protein - imported fish meal as a contrast, now has the following Advantages: Pig digestibility reaches 4120 kcal/kg, while imported fish meal is only 3600 kcal/kg; 30KG pig protein digestibility reaches 85%, which is equivalent to imported high-quality fish meal; Protein content of biological E protein 6065 is 60%, The amino acid composition is similar to that of fish meal, with 4.5% for lysine and 1.5% for methionine. Contains probiotics, enzyme preparations, biological peptides, vitamins, organic acids, bacterial polysaccharides, oligosaccharides, biological trace elements and a variety of biological metabolites, can prevent animal diseases, improve feed conversion, improve animal production performance, improve Livestock product quality, reduce environmental pollution and other important effects. Fishmeal does not contain these substances and does not have the above-mentioned effects. Biological E protein 5055 price is 3800 yuan / ton, 6065 price is 4,500 yuan / ton, imported high-quality fish meal price is 5400 yuan, domestic pure fish meal price is 4,800 yuan. The cost-performance ratio of biological E protein exceeds 30% of imported high-quality fish meal. 2. Replacement of all or part of the original biological components such as enzyme preparations, probiotics, acidifying agents, small peptides, and oligosaccharides in feedstuffs. A number of tests and comparative tests have shown that the content of the internal components of the biological E protein can fully meet the requirements for feeding. Requirements, bio-functional components after a reasonable configuration, the use of the effect is significantly better than a single biological product. Bio-E protein can provide high-quality protein, but also provide a rich, reasonable combination of bio-functional components, can directly reduce feed costs through effective substitution. 3. Make it possible to completely replace feeding antibiotics. Endangering feed safety, food safety and human health, and an important factor affecting the export of Chinese livestock products is the abuse of feed antibiotics in feeds, and feed antibiotics have become global public hazards. The result of a large amount of abuse of antibiotics has caused the rapid increase in the resistance of pathogenic bacteria and the decline of the animal's own immune function, which has caused the aquaculture industry in China to remain in a vicious cycle. Sooner or later, feed antibiotics have to withdraw from the historical stage of growth promoting and health promoting agents, and have become a global consensus. The main reason why some feed companies and farms insist on the use of feed antibiotics is that traditionally, feed antibiotics are used to prevent animal diseases from being reliable and difficult to replace, as to the role of promoting growth and improving feed conversion, etc. The reason for the reference. In recent years, the animal science and technology community has never stopped the study of alternative antibiotics for feed and has achieved fruitful results. Taking animal microecological preparations, enzyme preparations, biological peptides, Chinese herbal medicines, and polysaccharides as representatives, it has succeeded in the production of export-oriented animal husbandry and domestic safe meat, egg, and milk breeding. An important factor constraining the overall promotion of these bio-safety products in the entire feed industry and aquaculture industry is the high cost. The advent of biological E protein products has solved these two problems very well: the effective combination of multiple biological functional components has made the effect of promoting growth and health promotion more conclusive and stable; the industry conditions of scale, standardization, modernization and advanced research and development The concept and strong scientific research capacity have greatly reduced the production cost, making the biological E protein cheap and excellent value for money. 4. Properly reduce the content of protein and energy in feed, and reduce the cost of feed without reducing the quality of feed and without affecting the use effect. Full indicator tests and experimental results show that the biological E protein not only contains high energy levels and high quality protein itself, but also significantly increases the digestion and absorption rate of other nutrients in the feed and increases the energy protein level of the feed. 10%. Therefore, the biological E protein can be used to increase the intrinsic value of the feed, and it can also be used to appropriately reduce the feed cost. 5. Bio-E protein is suitable for the production of export-oriented animal products and green meat, eggs and milk foods. Bio-E protein itself is safe, no residue, no pollution, pollution-free green products, on the other hand, biological protein E can replace pollution source feed antibiotics, hormones, arsenic preparations and other chemical compounds with many biological functions. These pollution sources play a role in preventing animal diseases, promoting animal growth and improving production performance. Bio-E protein can also improve the quality of livestock products, improve the appearance and flavor of livestock products, and enhance the competitiveness of Chinese animal products in the international market. At the request of some export companies, Polaris Lilai also specially developed biological E protein products containing only microbial proteins and plant proteins to protect the export needs of livestock products. 6. The application of biological E protein to aquatic animals not only plays an important role in promoting weight gain, raising feed coefficients, improving survival rate, but also reducing the excretion of fecal harmful substances. After the beneficial bacteria enter the water body with the feces, Stabilize water quality, increase dissolved oxygen, reduce ammonia nitrogen and nitrite, purify aquaculture environment, inhibit the reproduction of pathogenic microorganisms, etc., reduce water exchange or even change water, and reduce breeding costs. In response to the feed characteristics of squid and soft-shelled turtle, Polaroid also developed a non-amylase bio-E protein product to ensure that the bait's stickiness is not affected.
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