First, the correct choice of molecular weight cut off
The molecular weight cut-off is selected based on the molecular weight of the macromolecules reserved within the membrane and the molecular weight of the small molecule contaminants to be removed. In order to achieve a reasonable and effective separation, the ratio of the molecular weights of the two substances to be separated is at least 25.
A rule of thumb for selecting the molecular weight cut off: the cutoff molecular weight value is chosen to be about half of the molecular weight of the macromolecule to be retained to achieve a retention of at least 90%.
Second, the correct choice of flat width
The choice of the flat width of the dialysis bag depends on the sample volume and dialysis capacity. Smaller dialysis tubes are faster to dialysis; larger dialysis tubes are slower to dialysis due to longer diffusion distances. For ease of use, it is recommended to use a dialysis bag with a total length (including closure clips and headspace) of approximately 10-15 cm.
Third, the chemical compatibility of the dialysis bag
Chemical compatibility is primarily used as a guide to use and is not a guarantee of chemical compatibility. Changes in temperature, concentration, long-term exposure, etc. may affect Use of the product. It is recommended that you set your own conditions for testing.
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