First, grasp the spawning time
Bullfrogs usually spawn more at 4 o'clock in the morning. No eggs are laid on rainy days, but on the second and third days after the rain, bullfrogs spawn a lot and fertilize.
Second, choose a good collection site
Bullfrogs generally like to lay eggs in waters with clean water, few humus, and near-neutral pH. Therefore, when picking eggs, it is necessary to pay attention to picking eggs in the waters with the above conditions.
Third, the collection method
After finding the eggs, first take some water with plants, and put them into pre-prepared barrels, washbasins, or large plastic bags. Then use a spoon to gently remove the eggs and move them into the barrels, pots, and bags. Keep the original direction when moving in. The front (animal polar hemisphere) is facing upwards and the opposite (vegetable hemisphere) is facing downwards.
Fourth, hatching method
Transfer the collected eggs to the prepared hatch pool. The hatch pool is generally 60-150 cm square with a water depth of 15 cm. The bottom of the pool is covered with 6 cm of sand, and some aquatic plants or duckweed are placed in the water. 6000 eggs per square meter. As long as the water temperature is suitable, larvae can be hatched every 2-4 days. Take a sexually mature male frog, remove the testes by caesarean section, place in a petri dish, add about 5 ml of clean pool water or well water (avoid using fresh tap water or oily water), and use a tweezers to grind the testes to make In the testis, swim to the water to make a suspension. Let sit for a few minutes. When fully active, squeeze the frog eggs into the Petri dish and gently shake the dish to spread the eggs one by one. At a temperature of 24°C, about 10 minutes after fertilization, most of the egg's animal hemispheres flipped up and the water in the petri dish appeared black. Whether the egg is over or not can be used as a sign of fertilization. After the eggs are overturned, they should be dumped and replaced with fresh water to provide sufficient oxygen to meet the need for further development of the fertilized eggs. Change the water once or twice a day until you have hatched. Or hold the right male and female frogs into a glass jar or washbasin with a 10 cm water depth in pond water. The pH of the water is about 7 and it is capped. After the ovulation is done, the male and female frogs are asked to leave in time. Egg hatching can also be done by manually placing the male and female frogs individually in the spawning pool to allow them to lay eggs. Spawning pond area of ​​2 square meters, depth of 0.35-0.40 centimeters. Postmenopausal bullfrogs are generally synchronized. After about 50 hours, the male and female frogs will have spawning, so that the fertility rate of the output is above 90%.
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