How to choose the appropriate β-Actin reference antibody?

How to choose the appropriate β-Actin antibody?

Nowadays, there are many choices for internal reference antibodies in the market, but it is also necessary to follow certain principles. How to find β-Actin antibodies suitable for your own experiments?

First, we need to consider the size of the protein of interest. We recommend that the internal reference should have a molecular weight difference of more than 5KD from the target protein to be detected. Therefore, you need to select the appropriate internal reference according to the molecular weight of the protein for your purpose, due to the molecular weight of β-Actin. It is approximately 42 kD, so the internal reference antibody is not well suited for use in WB assays for the detection of 38 kD-48 kD size target proteins.

In addition, although β-Actin is one of the most suitable internal reference antibodies, under certain conditions, some factors may also cause changes in the β-Actin content between samples. It is important to note that in adipose tissue, the expression level of β-Actin is very low and is not suitable as an internal reference.

Very durable, the quality is also very stable, put it for a year after purchase, take out the potency is still very high, and later bought their large package, directly customized 1mg powder, it is recommended. ——One client of the School of Life Science and Bioengineering, Beijing University of Technology

In addition, we also need to consider our own needs, especially the needs of experimental applications. In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources generally have better specificity, stability and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl β-Actin antibody (if the dilution ratio of WB detection is 1:5000, the final use solution is equivalent to 500 ml), 1 ml of β-Actin antibody with lower potency (if the dilution ratio of WB detection) It is 1:200, and the final use solution is equivalent to 2ml). Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.

Why choose to recommend the Abbkine beta-Actin antibody?

The β-Actin antibodies currently available on the market are either domestically packaged or natively produced; they are also imported, such as Abcam, Celll Signaling (CST), Sigma, Millipore, and the like. The domestically packaged β-Actin antibody is insufficient in quality stability, and its valence is also lost after being packaged. Some of the HA antibodies originating in domestic laboratories have better detection activity, but there are gaps in the type of application and it is difficult to control the stability between batches. Among the imported brands, Abcam's four-star recommended beta Actin antibody (ab8229) antibody 100μl is quoted at ¥3885 in 2011; Sigma's A5441 200ul is quoted at ¥4412; similarly, CST's beta-Actin antibody (#4967) is quoted at 100μl Need ¥2997, compared with other brands mentioned above, there is a certain price advantage.

If you only want to buy a β-Actin reference antibody with a higher cost performance and an imported brand, then Abbkine may be one of your important choices. We recommend Abbkine's β-Actin antibody (Cat. No. A01010). The immunogen used in this antibody is a KLH-conjugated Beta Actin N-terminal polypeptide, which recognizes the Beta Actin protein of species such as human, mouse, rabbit, rat, monkey, dog, chicken, pig and sheep. However, its price is only 60% of CST, less than half of Abcam, and the price/performance ratio is very obvious. As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing.

A high titer antibody not only affects the sensitivity of the experimental results, but also means a higher price/performance ratio. Abbkine's β-Actin internal reference antibody (Cat. No. A01010) In the conventional WB experiment, the recommended initial dilution ratio is 1:1000. Actually, in Abbkine application, there is a dilution ratio of 1:4000 or higher, which needs to be based on The customer's specific experimental conditions are optimized. In addition, Abbkine also provides HRP-conjugated β-Actin internal reference antibody.

Abbkine also offers a large package of 1ml β-Actin internal reference antibody, which is ideal for routine laboratory testing of immunological experiments. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the national free electric relay 801/802/803 or 027-84454412/4876 to contact Ai Meijie Technology Co., Ltd. for related product information and complete experimental solutions.

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