ACE® UltraCoreTM
Ultra-inert solid core column with ultra-inert solid core particles with extended pH stability
Also known as Fused-Core®, core-shell, SPP or nuclear reinforcing particles
Use selectivity by adjusting pH <br> Application# 1801
Column: ACE UltraCore 2.5μm SuperC18, 50 x 2.1mm
Samples: 1) atenolol 2) methylphenyl sulfoxide 3) physostigmine 4) prilocaine 5) bupivacaine 6) tetracaine 7) 1,2,3,4-tetrahydro- 1-naphthol 8) Carvedilol 9) Nitrobenzene 10) Mediazine 11) Amitriptyline 12) Phenylpentanone Temperature: 40 ° C
Flow rate: 0.60Ml/min
Wavelength: 254nm
Gradient: 3 – 100% B in 5 minutes B
Acidic mobile phase: A: 20 mM ammonium formate (dissolved in water) (pH 3.0) B: 2 mM ammonium formate (pH 3.0) (dissolved in 90:10 v/v acetonitrile/water)
Alkaline mobile phase: A: 0.1% NH3 (= 18 mM) (dissolved in water) (pH 10.7) B: 0.1% NH3 (= 18 mM), pH 10.7 (dissolved in 90:10 v/v acetonitrile/water)
Package Bonding Technology (EBTTM) for improved chromatographic analysis and stability
ACE UltraCore SuperC18 and SuperPhenylHexyl (phenyl-hexyl) phases are manufactured using our unique Encapsulation Bonding Technology (EBTTM).
This technique significantly increases the ligand coverage of the silica surface, effectively eliminating the adverse effects of unbonded silanol groups.
Higher ligand coverage results in improved inertness, chromatographic performance and stability.
ACE® UltraCoreTM Stationary Phase virtually eliminates the adverse effects of silanol on UHPLC and HPLC separations
Column inertia comparison
Column size: 50 x 2.1mm
Samples: 1) uracil 2) pyridine 3) phenol mobile phase: 30:70 (v/v) methanol/10 mM NH4OAc (dissolved in water) (pH 5.8) Flow rate 0.20 ml/min
Temperature: 22 ° C
Wavelength: 254nm
The comparison data does not represent all applications, and the reprint has been approved by the Open University of the United Kingdom.
Product availability and specifications
ACE UltraCore columns are designed for LC/MS compatible buffers.
To further extend the life of UHPLC and HPLC columns, it is recommended to use an ACE pre-column filter for HPLC column connections up to 5000 psi. PEEKTM hand tight fittings (p/n ACE-CC10) are recommended.
Reusable connectors are recommended for UHPLC column connections up to 25,000 psi (p/n EXL-CC10)
ACE UltraCore 2.5μm SuperC18 UHPLC/HPLC Column (UHPLC/HPLC hardware specification with 1000 bar/15000 psi pressure limit)
ACE UltraCore 5μm SuperC18 UHPLC/HPLC column (UHPLC/HPLC hardware specification with 1000 bar/15000 psi pressure limit)
ACE UltraCore 5μm SuperPhenylHexyl UHPLC/HPLC column (UHPLC/HPLC hardware specification with 1000 bar/15000 psi pressure limit)
ACE® is a registered trademark of Advanced Chromatography Technologies Limited. ACE® UltraCoreTM, SuperC18TM, SuperPhenylHexylTM and EBTTM are trademarks of Advanced Chromatography Technologies Limited. Advanced Chromatography Technologies Limited recognizes registered and unregistered trademarks of Advanced Materials Technology Inc., Phenomenex Inc., Thermo Fisher Scientific, Victrex Plc, and Waters Corporation, and has no affiliation with these companies. ACE authorized the national distributor, Philo is fully responsible for the sales and after-sales service of ACE series products in the Chinese mainland market.
Ultra-inert solid core column with ultra-inert solid core particles with extended pH stability
- 2.5μm and 5μm ultra-pure solid (superficially porous) particles
- Monodisperse particle distribution combines high efficiency with low pressure
- UHPLC column efficiency and performance on HPLC instruments
- Two bonded phases provide complementary selectivity for fast, systematic method development
- Excellent reproducibility and column life
- Ultra-inert phase ensures excellent peak shape
- Explore selectivity changes at low, medium and high pH conditions
- Designed for use with LC/MS compatible buffers
- Recommended pH range 1.5 – 11.0
Also known as Fused-Core®, core-shell, SPP or nuclear reinforcing particles
Use selectivity by adjusting pH <br> Application# 1801
Column: ACE UltraCore 2.5μm SuperC18, 50 x 2.1mm
Samples: 1) atenolol 2) methylphenyl sulfoxide 3) physostigmine 4) prilocaine 5) bupivacaine 6) tetracaine 7) 1,2,3,4-tetrahydro- 1-naphthol 8) Carvedilol 9) Nitrobenzene 10) Mediazine 11) Amitriptyline 12) Phenylpentanone Temperature: 40 ° C
Flow rate: 0.60Ml/min
Wavelength: 254nm
Gradient: 3 – 100% B in 5 minutes B
Acidic mobile phase: A: 20 mM ammonium formate (dissolved in water) (pH 3.0) B: 2 mM ammonium formate (pH 3.0) (dissolved in 90:10 v/v acetonitrile/water)
Alkaline mobile phase: A: 0.1% NH3 (= 18 mM) (dissolved in water) (pH 10.7) B: 0.1% NH3 (= 18 mM), pH 10.7 (dissolved in 90:10 v/v acetonitrile/water)
Package Bonding Technology (EBTTM) for improved chromatographic analysis and stability
ACE UltraCore SuperC18 and SuperPhenylHexyl (phenyl-hexyl) phases are manufactured using our unique Encapsulation Bonding Technology (EBTTM).
This technique significantly increases the ligand coverage of the silica surface, effectively eliminating the adverse effects of unbonded silanol groups.
Higher ligand coverage results in improved inertness, chromatographic performance and stability.
ACE® UltraCoreTM Stationary Phase virtually eliminates the adverse effects of silanol on UHPLC and HPLC separations
Column inertia comparison
- Solid column from leading manufacturers
- Comparison of the efficiency of basic molecular pyridine
- Efficiency measured at 50% peak height
Column size: 50 x 2.1mm
Samples: 1) uracil 2) pyridine 3) phenol mobile phase: 30:70 (v/v) methanol/10 mM NH4OAc (dissolved in water) (pH 5.8) Flow rate 0.20 ml/min
Temperature: 22 ° C
Wavelength: 254nm
The comparison data does not represent all applications, and the reprint has been approved by the Open University of the United Kingdom.
Product availability and specifications
| Particle size ( μm ) | Aperture ( Å ) | Surface area | Carbon load | Maximum pH range | USP | |||
| Phase functional group | ( m 2 /g ) | (%) | List | |||||
| Octadecyl in ACE UltraCore 2.5 SuperC18 package | 2.5 | 95 | 130 | 7.0 | 1.5-11.0a | L1 | ||
| ACE UltraCore 2.5 Packaged phenyl-hexyl | 2.5 | 95 | 130 | 4.6 | 1.5-11.0a | L11 | ||
| Octadecyl Group in ACE UltraCore 5 SuperC18 Package | 5 | 95 | 100 | 5.4 | 1.5-11.0a | L1 | ||
| ACE UltraCore 5 SuperPhenylHexyl (phenyl-hexyl) encapsulated phenyl-hexyl | 5 | 95 | 100 | 3.6 | 1.5-11.0a | L11 | ||
ACE UltraCore columns are designed for LC/MS compatible buffers.
To further extend the life of UHPLC and HPLC columns, it is recommended to use an ACE pre-column filter for HPLC column connections up to 5000 psi. PEEKTM hand tight fittings (p/n ACE-CC10) are recommended.
Reusable connectors are recommended for UHPLC column connections up to 25,000 psi (p/n EXL-CC10)
ACE UltraCore 2.5μm SuperC18 UHPLC/HPLC Column (UHPLC/HPLC hardware specification with 1000 bar/15000 psi pressure limit)
| 20mm | 30mm | 35mm | 50mm | 75mm | 100mm | 125mm | 150mm | |||||||||||||||
| 2.1mm | CORE-25A-0202U | CORE-25A-0302U | CORE-25A-3502U | CORE-25A-0502U | CORE-25A-7502U | CORE-25A-1002U | CORE-25A-1202U | CORE-25A-1502U | ||||||||||||||
| 3.0mm | CORE-25A-0203U | CORE-25A-0303U | CORE-25A-3503U | CORE-25A-0503U | CORE-25A-7503U | CORE-25A-1003U | CORE-25A-1203U | CORE-25A-1503U | ||||||||||||||
| 4.6mm | CORE-25A-0246U | CORE-25A-0346U | CORE-25A-3546U | CORE-25A-0546U | CORE-25A-7546U | CORE-25A-1046U | CORE-25A-1246U | CORE-25A-1546U | ||||||||||||||
ACE UltraCore 5μm SuperC18 UHPLC/HPLC column (UHPLC/HPLC hardware specification with 1000 bar/15000 psi pressure limit)
| 20mm | 30mm | 35mm | 50mm | 75mm | 100mm | 125mm | 150mm | 250mm | |||||||||||||||||
| 2.1mm | CORE-5A-0202U | CORE-5A-0302U | CORE-5A-3502U | CORE-5A-0502U | CORE-5A-7502U | CORE-5A-1002U | CORE-5A-1202U | CORE-5A-1502U | CORE-5A-2502U | ||||||||||||||||
| 3.0mm | CORE-5A-0203U | CORE-5A-0303U | CORE-5A-3503U | CORE-5A-0503U | CORE-5A-7503U | CORE-5A-1003U | CORE-5A-1203U | CORE-5A-1503U | CORE-5A-2503U | ||||||||||||||||
| 4.6mm | CORE-5A-0246U | CORE-5A-0346U | CORE-5A-3546U | CORE-5A-0546U | CORE-5A-7546U | CORE-5A-1046U | CORE-5A-1246U | CORE-5A-1546U | CORE-5A-2546U | ||||||||||||||||
ACE UltraCore 5μm SuperPhenylHexyl UHPLC/HPLC column (UHPLC/HPLC hardware specification with 1000 bar/15000 psi pressure limit)
| 20mm | 30mm | 35mm | 50mm | 75mm | 100mm | 125mm | 150mm | 250mm | |||||||||||||||||
| 2.1mm | CORE-5B-0202U | CORE-5B-0302U | CORE-5B-3502U | CORE-5B-0502U | CORE-5B-7502U | CORE-5B-1002U | CORE-5B-1202U | CORE-5B-1502U | CORE-5B-2502U | ||||||||||||||||
| 3.0mm | CORE-5B-0203U | CORE-5B-0303U | CORE-5B-3503U | CORE-5B-0503U | CORE-5B-7503U | CORE-5B-1003U | CORE-5B-1203U | CORE-5B-1503U | CORE-5B-2503U | ||||||||||||||||
| 4.6mm | CORE-5B-0246U | CORE-5B-0346U | CORE-5B-3546U | CORE-5B-0546U | CORE-5B-7546U | CORE-5B-1046U | CORE-5B-1246U | CORE-5B-1546U | CORE-5B-2546U | ||||||||||||||||
ACE® is a registered trademark of Advanced Chromatography Technologies Limited. ACE® UltraCoreTM, SuperC18TM, SuperPhenylHexylTM and EBTTM are trademarks of Advanced Chromatography Technologies Limited. Advanced Chromatography Technologies Limited recognizes registered and unregistered trademarks of Advanced Materials Technology Inc., Phenomenex Inc., Thermo Fisher Scientific, Victrex Plc, and Waters Corporation, and has no affiliation with these companies. ACE authorized the national distributor, Philo is fully responsible for the sales and after-sales service of ACE series products in the Chinese mainland market.
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