The mother culture medium of the edible fungi is usually a PDA agar medium, and raw materials such as agar, magnesium sulfate, monohydrogen phosphate, glucose and potato, and glass test tubes are required for preparing the PDA medium. The cost is high, the preparation method is tedious, and Higher professionalism is not suitable for ordinary producers. After many years of practice, the author explored a mother culture medium made from rice, which is described below. Material rice, potassium dihydrogen phosphate, magnesium sulfate, small infusion bottles, etc. Preparation Method Preparation of nutrient solution: Take 10g of potassium dihydrogen phosphate, 5g of magnesium sulfate, dissolve in 1000ml of cold water; take proper amount of rice in a small infusion bottle, pay attention to add 1/3 of the bottle height, and pour it down. Into the nutrient solution, the nutrient solution is not over 1cm above the rice, and the tampon is plugged. A layer of kraft paper is wrapped around the tampon and sterilized in an autoclave. The sterilization is completed until the bottle temperature is reduced to about 25C. Inoculation. Using rice culture medium, the bacteria germinate quickly after the inoculation of bacteria, and they grow robustly. They have the rejuvenating effect. Each bottle of mother rice culture medium can be used to expand the original species of 40 to 50 bottles, which is suitable for large-scale propagation of the original species. It can also be used. As preservation bacteria, and the production process is simple, easy to get raw materials, low cost, very suitable for ordinary edible mushroom growers.
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