CyTOF® Mass Spectrometry Flow Cytometry – The Top 10 Innovations in 2011 by The Scientist.
CytoF mass spectrometry, using metal element markers (usually metal-labeled specific antibodies or dyes) to label or recognize signal molecules on the cell surface and inside, then separate individual cells by flow cytometry, and then inductively coupled plasma Mass spectrometry (ICP-MS) is used to observe the atomic mass spectrum of individual cells. Finally, the atomic mass spectrum data is converted into signal surface data on the cell surface and inside, and the obtained data is analyzed by professional analysis software to achieve the cell phenotype. And fine observation of the signal network.
CytoF mass spectrometry, using metal element markers (usually metal-labeled specific antibodies or dyes) to label or recognize signal molecules on the cell surface and inside, then separate individual cells by flow cytometry, and then inductively coupled plasma Mass spectrometry (ICP-MS) is used to observe the atomic mass spectrum of individual cells. Finally, the atomic mass spectrum data is converted into signal surface data on the cell surface and inside, and the obtained data is analyzed by professional analysis software to achieve the cell phenotype. And fine observation of the signal network.
This study, through a method that can be called a large number of cell counting methods, observed 34 substances in different morphological cells and surfaces produced by human bone marrow , which not only correctly classified more than 10 different types of immune cells, but also observed various types of immune cells internal changes, which predict the changes that may occur. This will help to measure the response and efficacy of prescription drugs on human cells faster and more extensively , detect cytopathic effects in advance , and develop therapeutic drugs for individuals .
references:
Bendall, SC, et al., Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.
Science. 332 (6030): p. 687-96.
Http://
Read the original text: http://
Bendall, SC, et al., Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.
Science. 332 (6030): p. 687-96.
Http://
Read the original text: http://
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