In the fields of biological research, agricultural products and seed inspection, and inspection and quarantine of import and export commodities, it is often necessary to grind plant tissues to obtain biological macromolecules such as DNA , RNA or protein for subsequent analysis and detection. With the accelerating progress of laboratory research, manual grinding has been unable to meet the needs of scientific research. More and more researchers have begun to use the plant tissue grinder for grinding, which not only saves time and effort, but also is simple and efficient. Among them, the most popular grinding machines are based on the principle of high-speed collision of grinding steel balls. The most common ones are mainly two types, one is horizontal plant tissue grinder, and the representative type is DHS TL series automatic tissue grinder produced by a company . The other type is the vertical grinding machine, which is more representative of a US brand tissue grinding machine. So what are the performance characteristics of these two types of machines? Is there any difference in use? What are the differences in grinding results? How do you choose a model from the experiment? This article compares the above two representative models for reference, and makes a brief summary of these issues:
Horizontal plant tissue grinder | Vertical grinding machine | |
Model features: | · The rocker arm is connected horizontally with the motor, and the grinding bead is horizontally reciprocating arc-type random collision; · Maximum oscillation rate: 2000 rpm ; · Maximum throughput 2 × 96- well deep well plate or 2 × 50ml grinding tank; · The height of the instrument is about 200mm ; ·Programmable intermittent grinding | · The rocker arm is vertically connected to the motor, and the grinding beads are vertically reciprocating single point collision; · Maximum oscillation rate: 2000 rpm ; · Maximum flux 4 × 96 hole deep well plate; · The height of the instrument is about 520mm ; · Does not support intermittent grinding |
Use difference: | · Generally operate on the lab desktop · Freeze grinding ( condensed water will not immerse into the shaft ) ·The adapter is fixed with a knob and a locking device. | · Generally put on the ground to operate · Does not support freeze grinding ( some brand support , but condensate is easy to seep into the shaft ) ·The adapter is clamped up and down |
Grinding effect: | ·The discharge particle size is about 5 μ m | ·The discharge particle size is about 5 μ m |
So how do you choose the model that suits you?
Reasons for vertical selection: more than 200 micro-samples are required for one grind , or ultra-large ( 50-100 ml ) samples are processed at one time; no cryo-grinding is required.
Choice of horizontal reasons: used to operate on the laboratory table; one treatment volume below 200 micro-samples, while grinding a large volume ( about 50ml ) or hard samples; need to carry out frequent freeze grinding;
Regarding the price, as far as the information is currently known, the price of the domestic horizontal plant tissue grinder and the vertical grinding machine is not much different (the former average price is slightly more expensive), and the two have different models of the machine, so the pricing is not First, the market price is generally around 30,000-60,000. Compared with similar imported models (mainly horizontal), the price is more than 100,000 yuan. In fact, regardless of technology and quality, the gap between domestic and imported grinding machines is very small. In the spot and after-sales, domestic grinding machines have obvious advantages. After all, the long delivery time and high maintenance costs are eliminated. Users like it. And because of patriotism, the author is also more inclined to choose domestic machines. Of course, the final choice of horizontal or vertical must be combined with the actual needs of the laboratory and the specific budget.
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