Release date: 2016-07-14
It is understood that researchers at Organovo and Roche Pharmaceutical's research and early development departments are using Organo's 3D printed human liver tissue to create an analytical model for drug-induced liver damage and to distinguish between trovafloxacin and levofloxacin. (levofloxacin), these two closely related compounds have different toxicity characteristics.
The development of 3D printing human organs is currently a very important frontier medical research field. Although the field is still in its infancy, many scientists hope that in the not too distant future, patients will be able to obtain artificial organs to replace defective liver, kidney and heart. In fact, today's 3D printed human tissue has begun to play a very important role in medicine. Using 3D bioprinting organizations, researchers in the pharmaceutical industry can now test the possible effects of drugs on "human" organs without the need for human volunteers. This allows drug developers to conduct more aggressive experiments without regard to the life of the test subject.
It is understood that as the leading 3D bioprinting company. Organovo has been providing artificially manufactured organizations for drug research for several years. A new study by Organovo and pharmaceutical giant Roche showed that Organovo's 3D printed human liver tissue can be used to successfully distinguish between multiple substances with different levels of toxicity. This ability can help pharmaceutical companies identify substances that may have harmful effects on the human body.
It is reported that using Organovo's 3D bioprinting human liver tissue, researchers were able to detect the toxicity of troxafloxacin, a third-generation anti-infective drug, because a patient was found to have taken the drug one year after the market. He died of liver poisoning and was delisted. When the drug was approved by the regulatory authorities, the traditional test method failed to identify its toxicity. Today, using 3D printed human liver tissue, researchers at Roche and Organovo are able to distinguish the toxicity characteristics of troxacin and levofloxacin, a structurally related but non-toxic compound.
Organovo hopes that the function of these experiments will be to combine 3D printed liver tissue with other methods, such as animal experiments and 2D cell culture models, to pave the way for future testing of pharmaceutical products. 
Histological effects of troxacin on 3D bioprinted liver tissue. Single arrows indicate cell adhesion loss and double arrows indicate increased stem cell necrosis.
It is reported that Organovo's 3D printed human liver tissue is composed of the patient's parenchymal (liver) and non-parenchymal (endothelial and hepatic stellate cells) cell populations, both of which have a certain spatial structure and three-dimensional shape. The three-dimensional nature of the tissue allows researchers at Roche and Organovo to identify intercellular connections, CD31+ endothelial networks, and the difference between desmin positive, smooth muscle actin-negative static stellate cells.
In addition, the 3D printed tissue in the culture medium can maintain the metabolism of ATP and albumin at a relevant level for more than four weeks and maintain the drug-induced enzymatic activity of cytochrome P450 - this is 2D cell culture. Unable to achieve.
“Organovo's bioprinting technology creates an in vitro system consisting of multiple types of stem cells in a defined spatial structure that can be used over time to collect histopathological and biochemical data for clinical toxicity. Experiment.†Dr. Sharon Presnell, Chief Scientific Officer of Organovo, said: “Our long-lasting, reproducible models can also be used to measure specific responses to cell types and explore mechanisms of toxicity to develop alternative solutions.â€
The results of this study were published in the July 7 issue of PLOS One, entitled "Bioprinted 3D primary liver tissue for organ-level assessment of clinical drug toxicity in vitro (Bioprinted 3D primary liver tissues allow assessment of organ-level response to clinical drug induced toxicity in vitro).
Source: Tiangongshe
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