Rapid Determination of Germination Rate of Wheat and Corn by Staining Method

The germination rate of wheat and corn seeds is quickly determined by dyeing method in production. The specific methods are as follows:

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100 capsules of corn seeds and 200 seeds of wheat seeds were selected and soaked in warm water of 30 ° C ~ 35 ° C for 6 hours to 8 hours to fully inflate the seeds. 100 pieces of the inflated seeds were taken and cut into two halves along the midline of the embryo with a blade, half of which was used for the measurement. Place the prepared half seeds in a cup and add a red ink solution diluted 20 times to immerse the seeds. After 15 to 20 minutes of dyeing, remove the seeds, rinse the seeds with water, and wash off the color. Observe the coloration of the seed part after rinsing. If the embryo is not colored or slightly reddish, it is a viable seed. If the embryo is dyed in the same deep red color as the endosperm, it is an inanimate seed. The percentage of live seeds is calculated in turn, which is the germination rate.

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