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The automatic enzyme-free workstation is an enzyme-free instrument that meets the requirements of medical laboratories. It integrates automatic reagents, incubation, washing, and results. The whole process is controlled by computer, which realizes the automation and standardization of the operation process and overcomes the manual work. The limitations and cumbersomeness of the operation save manpower and time and greatly increase work efficiency. At the same time, accidental errors caused by certain human factors are avoided, and the precision, repeatability, sensitivity, and specificity of the test are improved. Of course, in order to ensure the accuracy of the analysis results and the normal operation of the instrument, to extend the service life of the instrument, and to ensure the smooth completion of daily work, it is necessary to pay attention to some details of the daily operation of the automatic enzyme-free workstation. Some experiences and conclusions.
1. About daily maintenance
Mainly refers to the pipe flushing when switching on and off, it must be flushed with a large amount of distilled water and the specified flushing liquid. Although this step is simple, it can not be omitted, because it can ensure the smoothness of each working channel, and smoothly carry out and extend the test. The life of the instrument is especially important.
2, about the cold start
Due to the busy work, process observations during cold start (ie, initialization) are often ignored. However, it is still recommended to pay attention to the following: whether the sound of the moving plate and the main conveyor is different (indicating whether there is looseness or no foreign matter), whether the pre-washing is dry and filled with water (tip pin) If there is any blockage in the hole, etc., the problem can be solved early and solved early, laying a good foundation for the smooth progress of the following work.
3, about project programming
How to make full use of the instrument, strive for time space, try to shorten the waiting time of the board and the board (including reducing the appearance of gray belt), how to carry out project programming is very important. The general purpose of the proposal is to work together and intersperse. For example, there are two fully automated enzyme-free workstations available, which should be considered for pre-programming the hepatitis program on one machine, and the other for testing including miscellaneous AIDS, HIV, Epstein-Barr virus and other miscellaneous items. When a machine has a gap, you should consider transferring an item from another busy machine to this. When programming, you should pay attention to the overall arrangement, the two-step test is programmed in the same area, and the one-step method is placed in the front or back area, so that the time and space of the instrument can be fully mobilized, and the idleness of the instrument can be reduced, thereby shortening Waiting time to improve work efficiency.
4, about the reaction plate
First, the rack should be handled before the sample is loaded, and the flat strip should be taken (to avoid the phenomenon of ejecting). In addition, after adding the sample, carefully observe whether there are blood clots or impurities in each sample. If there is any treatment, the plate should be moved (to avoid clogging the plated pinhole). In addition, when entering the test code of the reaction plate in the computer, you should "three check three pairs", and confirm that the load button is correct. In particular, the code of the reaction project, which instructs the instrument to add the corresponding reagent. Once the input is wrong, the entire test will be invalidated, which will delay the time and waste the reagent.
5, on the handling of certain minor faults
If the main conveyor belt is suddenly interrupted and automatically locked during operation, or the syringe of a certain reagent cartridge of the reagent cartridge is stuck in the gripping hand, interrupting the action of adding the reagent and automatically locking, it is not necessary to shut down and restart, and the operation can be performed. : The former manually pulls the main conveyor belt to the front position of the * incubator, and then unlocks it. The latter should be careful not to forcibly remove the syringe, but simply click "unlock" in the computer and continue to operate the instrument after the syringe has been reset.
In addition, the failure of the washing machine is also more common, usually caused by the blockage of the pinhole, generally after one or two unlocking flushes can return to normal. In this process, attention should be paid to the observation of the wash head in the self-test, whether a certain pinhole adheres to the blood clot. If so, use the instrument to open the gap of the wash head protection cover and quickly remove the clot with cotton or paper.
6, about the washing tank and washing station
Pay attention to the cleaning problem of the washing tank. When it is used up on the same day, the remaining liquid in the drum must be drained to avoid long bacteria. If there is leakage or no water, stop using it. The washing station should be kept as dry as possible, especially after shutting down, the water stain must be wiped with a rag.
7. Regarding the situation where no color or color difference occurs after the reaction plate zui appears
The cause should be immediately searched. After the reasons for non-reagents or specimens are excluded, the instrument can be started, comprehensive analysis, point-by-point inspection, point-by-point elimination, until the "cause" is found.
Generally, it should be considered from the following points: whether the preparation of the washing liquid is mixed with the acid solution; whether the temperature of the incubator is out of balance; whether the water in the washing machine is normal; whether the plate is not correct or the reagent in the reagent tank is not fully replenished in time. The result is an empty addition (leakage) reagent; is it due to the error entered in the item code just mentioned in point 4? Once the root cause of the problem is found, resolve it immediately so that the following tests can continue smoothly.
8. About reagent reagents are the basic conditions for conducting tests.
Its quality directly affects the test results after zui. Therefore, the following three points should be noted: the reagent tank should be replaced regularly, usually once a week. Before use, the reagent in the tank should be observed for the presence or absence of bacteria and mildew. If it is, it should be replaced immediately. After use, the reagent tray should be placed in time. People save the refrigerator.
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