Chinese scientists have made major breakthroughs in epigenetic research
September 20, 2016 Source: Chinese Journal of Science
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];The Gao Shaorong team of Tongji University first revealed the process of histone H3K4me3 and HK27me3 modification during mouse preimplantation embryo development from a genome-wide level, and found that wide H3K4me3 modification plays an important role in gene expression during preimplantation embryo development. Regulation. Related results were published online in Nature on September 15.
Gao Shaorong's research group used a very small number of cells to detect the changes of histone H3K4me3 and H3K27me3 modification in various stages of mouse preimplantation embryo development. These two modifications correspond to gene activation and silencing, respectively.
The researchers found that the establishment of histone H3K4me3 and H3K27me3 modifications were significantly different, H3K4me3 modification was established more rapidly, and it was preferred to establish a promoter region with higher CpG content and lower DNA methylation level, and the establishment of H3K27me3 modification. It is slower and tends to be established in a promoter region with a lower CpG content.
The most important finding of this time is to see that although the H3K4me3 modification is rarely completely established and removed after the 2-cell phase, the width of the H3K4me3 signal is constantly changing and there is a large amount of bread in the genome of the early embryo ( >5kb) H3K4me3 signal. This wide H3K4me3 signal is low in cell lines as well as in normal somatic cells. Importantly, these broad H3K4me3 signals are closely related to the high expression of genes and the developmental fate of cells. This indicates that in early embryos, H3K27me3 and other modifications have not been fully established, and the regulation of gene expression by cells may be There are completely different epigenetic regulatory mechanisms.
The study also found that the width of H3K4me3 modification gradually changes during embryonic development, indicating that the broad H3K4me3 modification is a regulated epigenetic modification that precisely regulates gene expression in various stages during early embryonic development, and may Play an important role in more physiological processes.
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