Tissue Culture Seedlings-Cultivation (hardening) Methods In general, the method of cultivating tissue culture seedlings is basically the same as the method of raising orchids. However, we must first understand the special environmental conditions of tissue culture seedlings. problem. The tissue culture seedlings are cultured under aseptic conditions. The culture flask is a heterotrophic organism. Humidity needs to be above 90%. Understand the above environmental conditions, I believe there is a certain help to cultivate tissue culture seedlings. The following is a brief description of the culture method of tissue culture seedlings: 1. The most important thing in the culture process is to completely prevent contamination. If it is a contaminated orchid, please do not bring it into the cultivation room. Fundamentally stop pollution sources from invading. 2. It is a good method to thoroughly sterilize cultured plant material. Use a drying oven or steam sterilizer to sterilize plant material and flower pots. Try not to use used pots and plant materials. 3, for small pliers, knives, scissors and other transplantation tools to do the fire disinfection or disinfection of drugs before use. 4, to ensure ventilation, fresh air. Like watering too much to absorb decay, bad air will rot. 5, the temperature of the culture room should be 25 ~ 28 °C (according to summer), preferably no more than 30 °C. 6. The relative humidity should be above 90%. 7. The cultured blue cells are weaker than wild blue and their growth frequency is also small. The connection between leaf and stem vascular bundles is very weak. Most organizations are weak and easily damaged. Just like looking after a child needs careful training. 8, belonging to heterotrophic organisms, very weak roots, a small number. It is recommended not to use organic fertilizer, apply inorganic fertilizer as far as possible, and apply diluted fertilizer at a prescribed ratio. 9, do not touch the orchid with your hand. Orchids are sensitive to salt concentrations. Don't touch the orchids with your hands. The salt on your hands is easily transferred to the orchids. 10. Use hair root promoters to promote the growth of new rhizomes and adapt to the environment. It is recommended that you always use the product. 11. When fertilizing the leaves, use a sprayer to fertilize it. The smaller the spray particles, the better. 12. When there is a small amount of plastic or orchid in the orchid room when the space is large, the Coke plastic bottle can be cut open on the flower pot to increase the humidity. Pay attention to the high humidity, which can easily cause pests and diseases. At this point, the temperature and the amount of light received should be reduced. I cultivate the orchid culture environment (each person's breeding methods and the environment is different, the following method is for reference only): 1, to ensure good ventilation (alternate use of 2 fans to ensure 24-hour operation) - the external strong wind is not good for orchids It is recommended to set the fan's wind force to maximum after watering. 2, the general temperature should be maintained at 25 ~ 28 °C (summer standard), autumn is 20 ~ 25 °C (20 °C or less than 30 °C on the orchid growth), winter as much as possible to maintain at 15 °C. 3. Fertilizer (I use HY-PEX-HYPONex, Magic-Magamp-K, and self-made vitality agent). The summer should prevail. Apply fertilizer once every three days on the back of the leaves. In addition, fertilize the entire pot when watering every week. Residue fertilizers may cause soft rot. Therefore, after fertilizing for 1 hour, wash the fertilizer on the leaves with water. When using granular fertilizer, dilute the fertilizer with water until it is completely thawed. Incompletely thawed fertilizer particles adhere to the surface of the orchid leaf and may cause the leaves to become soft (as if burnt with hot water). 4. Planting materials: The plant material we use is the Japanese planting stone. 5, according to the environment, the specific watering method is different. The standard that I use to manage orchids is to pour water once every three days (according to spring, summer, and autumn). 6, the humidity must be maintained at about 60%. If the humidity is not enough, sprinkle water on the blue room floor. When the absolute temperature is high or when light is received, it is not suitable for watering. I usually use a spray can to spray three times of water from the light to the late afternoon, and ventilation is very important for raising blue. 7. Sterilization: Spray the fungicide once a week. Generally, insecticides can be used once a month. Reference: I live in an apartment on the 8th floor of an apartment in Beijing, with a balcony on the hall (window on the east side) (usually lighted between 12:00 in the morning and 12 noon) and the same method used in the hut to cultivate the blue orchid and tissue culture seedlings. Matters needing attention: The most important thing is to first consider the natural environment and cultivation methods of orchids, and choose the proper method of raising orchids. If you have an automatic greenhouse, be especially ventilated. Even in an automatic greenhouse (with fans and multi-blade fans), the fans or multi-blade fans are not ventilated until a certain temperature is reached. The disadvantage of the automatic greenhouse is that when the set temperature cannot be reached, the fan or multi-blade fan does not operate, which easily leads to poor ventilation of the blue room. The survival rate of the general seed blue is less than 100%, not to mention the fact that the tissue culture seedling, which is cultivated in an artificial environment, needs to focus on cultivating the environment. The survival rate of tissue culture seedlings is also less than 100%. In general, it is not a problem that the experts of tissue culture seedlings feed more than 95%, but ordinary love orchids are very difficult to do, because they do not understand the tissue culture seedlings and easily kill the orchids. It is recommended that you first understand the characteristics of tissue culture seedlings before purchasing. In general, there is no problem if the hardening time reaches one month, but it is best for novices to buy tissue culture seedlings that have been hardened for more than 6 months. The shorter the hardening period of tissue culture seedlings, the harder it is to raise them, so the longer the hardening period, the higher the price.
Natural pigments are food pigments obtained from natural resources. Pigments extracted mainly from animal and plant tissues and microorganisms (cultures), in which vegetative colorants predominate. Natural pigments not only have the function of coloring food, but also have physiological activity. At present, there are 48 kinds of edible natural pigments approved to be used in China, commonly used chili red, beet red, monascus red, cochineal red, gaolianghong, sodium copper chlorophyllin, turmeric, gardenia yellow, carotene, algal blue pigment, cocoa pigment, caramel pigment and so on.
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