Has your cell line development encountered bottlenecks?
1. Frequently being plagued by “monoclonality†problems, it requires a lot of work verification and sometimes it is questioned;
2. The cloning and screening process is cumbersome and takes 2-3 months to complete;
3. The Titer test needs to be cultured for several weeks to proceed;
4. The expression level of the screened cell line is not high enough;
5. The correlation of each stage (96 wells, 24 wells, 6 wells, shake flasks, AMBR) was not well correlated.
Beacon single cell light guide system will help you break through the bottleneck and bring a new world!
1. Precise and flexible single cell operation
Video 1: Single cell precision operation
Beacon's unique photo-localization technology accurately transfers any single cell to the NanoPen (culture chamber) and sorts single cells or monoclonals of interest.
2. Control the monoclonal from the source
Video 2: Make sure single cells
Methods such as conventional limiting dilution require multiple rounds of operation and combine multiple methods to find and confirm monoclonality. Beacon can directly culture target cells without wasting time on unwanted cells. WYSIWYG ensures that each NanoPen has only one cell and automatically removes multiple cells that may be present, making it easy for a single round of Beacon to achieve 99.5% monoclonality.
Video 3: Export target clone
The process of importing, cultivating, testing, and exporting has an image record that generates a complete report and provides strong evidence for subsequent filings.
3. From excellent to excellent
It’s better to pick one in a hundred miles! Conventional methods generally screen only a few hundred clones. Beacon can intervene when cell diversity and survival rate are optimal after transfection, and easily screen thousands of clones to select cell lines with higher expression levels, thereby greatly reducing subsequent production costs. .
Comparison of different screening flux results
4. Because it is tiny, it is sensitive
Conventional single cells are placed in microtiter plates and cultured for several weeks, with tens of thousands of cells to ensure that the secreted proteins reach detectable levels, while Beacon can reach single-cell detection levels , such as IgG Titer detection of individual B cells.
5. Good correlation of expression
One of the pain points of conventional microplate screening is that the expression correlation of clones in various levels of the system is not good enough, such as 96-well plates, 24-well plates, 6-well plates, and shake flasks. This can seriously affect work efficiency, increase workload, and even lead to project redoing. Beacon can guarantee that the titer in the NanoPen of only 1 nanoliter is well correlated with the titer of AMBR on the Chip.
6. Significant time savings
In the conventional cell line development process, the cloning and screening process takes 2-3 months, and Beacon can be easily completed in 5 days .
7. What is Beacon?
Conventional cell research generally takes a cell population as a research object or uses a method similar to a needle in a haystack to find a single cell. The Beacon single-cell photoconductive system can directly manipulate and culture individual target cells from the beginning of the experiment without wasting time on unwanted cells . The system uses a unique Opto Electro Positioning technology combined with a novel nanofluidic design to provide an integrated and efficient research platform for all single cell-based applications and developments.
Beacon is widely used: Cell Line Development, Plasma B-cell Ab Discovery, Cloning, cell strain stability screening, antibody engineering, memory B cell antibody discovery, Gene editing, tumor immunotherapy, cell co-culture research, tumor microenvironment research, stem cell research, etc.
Beacon product introduction link (including animation display):
Http://?equipid=4214283
This issue only introduces the performance of Beacon in the development of monoclonal cell lines, more applications, so stay tuned~
8. About "Classic Creatures"
"Diaoao Bio" (Shanghai, Hong Kong, Taiwan) was established in 1994. For more than 20 years, it has been adhering to the world-class instrument manufacturers. The experts with rich R&D experience are technical support, and the professional maintenance team is backing the service. Focus on providing the most advanced hardware and software equipment and technical services for life sciences, pharmaceutical research and development and disease treatment.
The exclusive distributors of " Dia 's Bio" are: IncuCyte, Beacon, Gyrolab, Avatar, PlexBio, Certus, HighRes, etc.
For more information, please contact "Shanghai Dianao Biotechnology Co., Ltd."
Website:
Telephone (transfer to the marketing department)
Email:
Long press QR code to add WeChat public number "tekontech"
Tetanus Toxoid Vaccine,Toxoid Vaccine,Hep B Immune Globulin,Immunoglobulin Injections
FOSHAN PHARMA CO., LTD. , https://www.foshanpharma.com