Release date: 2018-03-15
As the basic genetic material of life, the precise editing and rapid detection of DNA has always been highly valued. In recent years, with the discovery and development of the CRISPR/Cas9 system, new hopes for gene therapy have rekindled; despite some potential security risks and certain ethical disputes, the CRISPR/Cas9 system is relatively accurate and efficient. Has begun to be applied to clinical research. At the same time, scientists have begun to introduce the CRISPR system into the field of rapid nucleic acid detection, and hope to bring breakthroughs to existing clinical diagnostic techniques.
"Cell Research" magazine published on March 12th the Institute of Advanced Innovation of Molecular Plant Science / Institute of Plant Physiology and Ecology of the Chinese Academy of Sciences Zhao Guoping research group entitled "Cas12a has both cis and trans single-strand DNA cleavage activity" (CRISPR - Cas12a has both cis- and trans-cleavage activities on single-stranded DNA). In this study, the authors systematically studied the cleavage properties of Cas12a for target single-stranded DNA and non-target single-stranded DNA, and proposed a molecular model for Cas12a cleavage of single-stranded DNA.
The study found that once Cas12a forms a ternary complex with crRNA and target DNA, the complex exhibits a strong "chaotic" activity and cleaves any single-stranded DNA in the system into fragments. Based on this discovery, researchers can easily develop relevant test kits to detect various target DNAs, enabling rapid, sensitive, low-cost, and simple clinical diagnostic purposes.
In recent years, the academic team of the Chinese Academy of Sciences, Zhao Guoping, has focused on the development and application of synthetic biology enabling technologies, developed a number of methods for DNA editing and detection, and actively promoted these methods for clinical research.
It is reported that the research has received funding from the Chinese Academy of Sciences' pilot project ("Artificial Methods for Artificial Chromosome Construction and Stable Genetics"), the Chinese Academy of Sciences Youth Innovation Promotion Association and the National Natural Science Foundation.
Source: Science Network
Canned Mixed Vegetables,Mixed Vegetables Canned,Mixed Vegetables Tin,Mixed Canned Vegetables
ZHANGZHOU TAN CO. LTD. , https://www.zztancan.com