(1) Watermelon stretches during high temperature and humidity. Watermelon stretches during the season of high temperature and humidity, plant nutrient growth is strong, reproductive growth is inhibited or artificial management can not keep up with the field caused by unlucky cover. Insufficient illumination, high soil moisture, reduced ventilating performance, etc., produce empty plants, and must be pinched in time, beat water to increase ventilation and light transmission performance, or pinch off in the main vine leaves 4~6. The main vine to the top to promote side vines sitting melon.
(2) Improper management of water and fertilizers. Partial application of nitrogen fertilizer infested. The ranch plants should be pinched at 1 to 2 knots after the emergence of the female flower, and the nutrient supply to the ovary will be retained. This will increase the ovary quality and allow the melons to sit as required. At the same time, during the sitting period, phosphorus and potassium fertilizers should be added to coordinate nutrient balance, control vegetative growth, and promote reproductive growth. For the thin plants caused by high temperature and drought, early fertilizer should be applied. The method is to dig 15 cm deep from the root side of the plant, and apply 10 kg urea per mu, 10 kg of potassium sulfate, chase the water and fertilizer in dry weather, graze in the rain, and water the plants. Depends on soil moisture status. After flowering and before setting melons, do not apply water and fertilizers so as to avoid reminding me of falling larvae.
(3) High temperature and drought during flowering. Drought causes male and female flowers to be stunted. Extreme drought in flowering stage causes male flowers to be stunted due to high humidity. Generally, 0.5% potassium dihydrogen phosphate solution or phosphorus fertilizer is used for watering roots, or water is used to change air and soil temperature. To improve the microclimate in the field and promote pollen development to increase fertility. Plants with no female flowers due to high temperature and drought. If the vines grow to 3 to 5 meters, if there are still no female flowers, they should be cut off immediately, 1.5 meters long for each vine, and then poured at a concentration of 0.5% potassium dihydrogen phosphate or Phosphorus solution, to be thoroughly irrigated, promotes new vines and leaves melons on new vines.
(4) Low temperature during rainy season. During the flowering period, rain prevention measures may be taken in advance according to the weather forecast. On the afternoon of the previous day, she went to the field to wear a rain-proof paper hat to the female flowers that were to be opened the next day. The male flowers to be opened in the field were picked and put into containers in the field at 5 o'clock in the morning the next morning. When the female flowers at 6-9 o'clock are open, use the open male pollen to gently apply to the stigma of the female flowers, wipe it evenly, and immediately put on a rain-proof paper cap to the female flowers after pollination to prevent female flowers and pollen from pollen caused by rain. Rupture, sperm nucleus loss, thereby affecting melons.
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PCR ( Polymerase Chain Reaction)
It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.
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